Tag

Part:BBa_K1125001

Designed by: Tsung-Yu, Lu   Group: iGEM13_NTU_Taiwan   (2013-09-16)

Tandem Affinity Purification (TAP) tag

used in protein detection or purification



Usage and Biology

Purification uses TAP tag is a synthetic construct composed of three fragments: calmodulin binding peptide (CBP), tobacco etch virus protease (TEV protease) cleavage site and Protein A


Western blot uses When used a protein tag, TAP tag can be specifically detected by anti-TAP antibody.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 215
    Illegal BglII site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None